![]() (Exposure times for TH, galanin, and GalR3 were approximately as stated above.) However, galanin mRNA is observed at this level just dorsal to the serotonin cells and extending dorsally/laterally in the vPAG. A comparison of the distribution of transcripts for TPH2, galanin, and GalR3 in the DRN/vPAG shows that TPH2 and GalR3 exhibit a distinct overlap, but no galanin mRNA can be seen in this particular subregion. A weak GalR1 signal was seen in the LC region expanding medially into the pontine central gray but did not seem to be associated with the pigment characteristic of the NA neurons as confirmed in cresyl violet-counterstained sections analyzed with bright- and dark-field microscopy (see ). GalR3 appears to be the predominant galanin receptor in the LC. ![]() ![]() Note that GalR3 transcript levels are fairly similar among individual neurons, as is the TH signal, in contrast to the high variability of galanin mRNA. ![]()
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